Tissue Culture — Homi Bhabha Class 9 Biology MCQs with Solutions
Free Homi Bhabha Class 9 Biology Tissue Culture MCQs with step-by-step solutions (13 questions). Part of Biotechnology & Applied Biology. Practise online on Prepizo — no login needed.
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Questions with solutions
Q1 — Tissue Culture · medium · theory
Tissue culture is a technique of growing cells, tissues or organs in a(n):
A. open field
B. artificial nutrient medium under sterile conditions ✓ Correct
C. salt water
D. ordinary pot of soil
Solution: Tissue culture grows plant/animal cells in a sterile nutrient medium in the laboratory.
Q2 — Tissue Culture · hard · theory
A small part of a plant taken for tissue culture is called a(n):
A. clone
B. gamete
C. explant ✓ Correct
D. embryo
Solution: An explant is the piece of tissue used to start a tissue culture.
Q3 — Tissue Culture · medium · theory
The plants produced by tissue culture from a single parent are:
A. seed-grown
B. always hybrids
C. genetically identical (clones) ✓ Correct
D. all different
Solution: Tissue-cultured plants are clones — genetically identical to the parent.
Q4 — Tissue Culture · hard · theory
Producing a large number of plants quickly from small tissues by tissue culture is called:
A. germination
B. pollination
C. fermentation
D. micropropagation ✓ Correct
Solution: Micropropagation rapidly multiplies plants from tiny explants.
Q5 — Tissue Culture · medium · theory
Tissue culture must be carried out under sterile (aseptic) conditions to:
A. reduce the yield
B. add more microbes
C. prevent contamination by microbes ✓ Correct
D. increase temperature
Solution: Sterile conditions stop bacteria and fungi from spoiling the culture.
Q6 — Tissue Culture · hard · theory
One major advantage of tissue culture is the production of:
A. weeds
B. more insects
C. disease-free plants ✓ Correct
D. polluted soil
Solution: Tissue culture can produce healthy, disease-free planting material.
Q7 — Tissue Culture · medium · theory
Tissue culture is widely used commercially to grow plants such as:
A. fish
B. only wild grasses
C. orchids and bananas ✓ Correct
D. bacteria only
Solution: Ornamental and commercial plants like orchids and bananas are mass-produced by tissue culture.
Q8 — Tissue Culture · hard · theory
Compared with growing plants from seeds, tissue culture allows:
A. only one plant per year
B. rapid multiplication of many identical plants in a small space ✓ Correct
C. no control over quality
D. slower and fewer plants
Solution: Tissue culture rapidly yields many uniform, high-quality plants in limited space and time.
Q9 — Tissue Culture · easy · theory
In plant tissue culture, the mass of undifferentiated cells that first grows on the nutrient medium from a piece of tissue is called the:
A. Callus ✓ Correct
B. Nodule
C. Clone
D. Explant
Solution: The unorganised, dividing mass of cells that forms on the medium is the callus. The explant is the original piece placed on the medium; a clone is the set of identical plants finally produced.
Q10 — Tissue Culture · easy · theory
Plants produced by tissue culture from a single parent are genetically identical to that parent. Such a group of identical plants is called a:
A. Mutant
B. Hybrid
C. Variety
D. Clone ✓ Correct
Solution: Because they arise by mitosis from one parent without fertilisation, all the plants are genetically identical and together form a clone. A hybrid results from a cross between two different parents.
Q11 — Tissue Culture · medium · theory
Tissue culture works because a single plant cell can grow into a complete plant. This ability of a cell to form a whole organism is called:
A. Fertilisation
B. Totipotency ✓ Correct
C. Transpiration
D. Turgidity
Solution: Totipotency is the capacity of a single cell to divide and differentiate into every cell type, forming a complete plant. It is the fundamental property that makes micropropagation possible.
Q12 — Tissue Culture · medium · theory
Tissue culture is always carried out inside sterilised glassware in an aseptic chamber. The main reason is that:
A. The tissue needs high pressure to grow
B. Bacteria and fungi would grow faster than the tissue and spoil the culture ✓ Correct
C. Sterile air adds nutrients to the medium
D. Sunlight is harmful to the cells
Solution: The nutrient-rich medium is an ideal food for microbes too. Any contaminating bacterium or fungus multiplies rapidly and overruns the slow-growing plant tissue, so strict sterile technique is essential.
Q13 — Tissue Culture · hard · numerical
A laboratory starts with 1 explant. In every subculture cycle the number of plantlets becomes 5 times the previous number, and one cycle takes 3 weeks. How many plantlets are present after 9 weeks?
A. 25
B. 15
C. 125 ✓ Correct
D. 45
Solution: 9 weeks is 3 cycles. Each cycle multiplies by 5, so the count is 5 × 5 × 5 = 5³ = 125. Choosing 15 comes from adding (5+5+5) and 25 from stopping at 5² after only two cycles.